What is Hemin ?
Hemin, a breakdown product of heme, plays a significant role in various physiological and pathological processes. From oxidative stress to iron metabolism, hemin levels are critical biomarkers in numerous studies related to neurodegenerative diseases, inflammation, and blood disorders .
Accurate and efficient detection of hemin is essential for scientists working in clinical diagnostics, drug development, and biomedical research . This is where advanced tools like the AffiAssay™ R Hemin Colorimetric Assay Kit come into play.
Why Hemin Detection Matters
Hemin has been implicated in several biological pathways, including:
- Oxidative stress regulation
- Inflammation modulation
- Apoptosis and cellular signaling
- Neurological disease progression
Improper hemin levels can lead to conditions such as porphyria, hemolytic anemia, and neuroinflammation . Therefore, measuring hemin with high precision is vital for both basic science and translational medicine.
Challenges in Hemin Measurement
Traditional methods of hemin detection often involve complex protocols, expensive equipment, or lack specificity. Researchers need a method that balances accuracy, sensitivity, and ease of use —especially when dealing with limited sample volumes or high-throughput screening.
This is where colorimetric assays shine due to their:
- High sensitivity
- Visual or spectrophotometric readouts
- Compatibility with microplate readers
- Cost-effectiveness
Introducing the AFF-0024 AffiAssay™ R Hemin Colorimetric Assay Kit
The AffiAssay™ R Hemin Colorimetric Assay Kit (Cat No. 117225) is designed for rapid and accurate quantification of hemin in biological fluids, tissue lysates, and cultured cells . With a detection range of 0.1–20 µM , this assay offers excellent sensitivity and reproducibility.
Key Features:
- Simple protocol : Mix-and-read format
- Fast results : Detect hemin within 30 minutes
- High specificity : Minimal interference from other heme derivatives
- Colorimetric detection : Absorbance at 400 nm
- Suitable for : Serum, plasma, urine, cell lysates, and more
Whether you're studying heme oxygenase activity , oxidative stress markers , or drug-induced toxicity , this kit provides a robust solution for hemin analysis in your lab.
Applications in Research
Researchers have successfully used this kit in studies involving :
- Neurodegenerative disease models
- Liver injury and drug metabolism
- Anemia and erythropoiesis
- Inflammatory response assays
Its reliability and flexibility make it ideal for both academic labs and pharmaceutical research settings.
How to Use the Kit Effectively
The kit includes all necessary reagents and a detailed manual. Here's a quick overview:
- Prepare samples and standards.
- Add working reagent to each well.
- Incubate for 10–15 minutes.
- Measure absorbance at 400 nm.
- Calculate concentration using standard curve.
📊 Example of a results table :
Concentration de Hémin (µM) | Absorbance @400 nm |
|---|---|
0 | 0.01 |
0.1 | 0.08 |
0.5 | 0.25 |
1 | 0.40 |
5 | 1.20 |
10 | 1.80 |
20 | 2.10 |
No specialized training or equipment beyond a basic microplate reader is required.
✅ Tips & Recommendations :
👩🔬 Wear gloves and protective eyewear.
💧 Use distilled or deionized water for all dilutions.
🕒 Perform the analysis quickly after sample preparation to minimize hemin degradation.
🧽 Clean the plate thoroughly after reading for reuse (if applicable).
Where to Buy the AFF-0024 AffiAssay™ R Hemin Colorimetric Assay Kit ?
You can purchase the AFF-0024 AffiAssay™ R Hemin Colorimetric Assay Kit ( CAT# AFG-BV-0704) directly from AffiAssay™. They offer reliable shipping, technical support, and competitive pricing for academic and industrial buyers.
Hemin detection is more than just a routine lab task—it’s a window into understanding complex biological systems. The AffiAssay™ R Hemin Colorimetric Assay Kit delivers the accuracy, speed, and simplicity modern researchers demand.
If you're looking for a cost-effective, reliable, and user-friendly hemin assay , consider adding this kit to your lab arsenal today.
🔗 Call-to-Action (CTA) :
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