🔬 Taking Control of Mycoplasma

🧬 The Hidden Threat of Mycoplasma Contamination in Cell Culture

Mycoplasma contamination is one of the most common—and most overlooked—problems in cell culture laboratories. These small bacteria lack a cell wall, making them resistant to common antibiotics and undetectable under light microscopes. Their silent presence can distort gene expression, compromise protein synthesis, and ultimately render your experimental data unreliable. That’s why proactive mycoplasma detection and removal is essential for any lab working with mammalian cells.

🧪 Step 1: Detect with the AffiZYME® MycoVisual Kit

The first line of defense is early detection. The AffiZYME® MycoVisual Kit is a rapid, LAMP-based colorimetric assay that screens for mycoplasma in just 60 minutes—no special equipment or DNA extraction required.

🔍 Why choose AffiZYME®?

• Color-based readout (blue to purple) for easy interpretation
• Closed-tube format prevents cross-contamination
• Ideal for weekly screening routines in any cell culture lab

🎯 Stop contamination before it spreads. Screen smart, screen fast.

🔗 Order the AffiZYME® MycoVisual Kit now

🧪 Step 2: Confirmation with AffiPCR® MycoDetect qPCR Kit

When your visual screening shows signs of contamination, confirm it with the AffiPCR® MycoDetect Kit. This real-time PCR assay offers ultra-sensitive mycoplasma detection, capable of identifying fewer than 10 copies of DNA. It works directly from the culture supernatant—no DNA extraction required. Built-in positive and negative controls ensure each run is reliable. This qPCR-based solution is ideal for critical applications, such as therapeutic protein production, stem cell lines, and vaccine development.

🎯 Stop contamination before it spreads. Screen smart, screen fast.

🔗 Order the AffiPCR® MycoDetect qPCR Kit now

🧼 Step 3: Eliminate with AffiCLEAN® MycoX Removal Mix

Detection is only half the battle—elimination matters most. The AffiCLEAN® MycoX Removal Mix provides a non-toxic, highly effective solution to eradicate mycoplasma while preserving cell viability.

Why AffiCLEAN® MycoX ?

• Removes over 70 species of mycoplasma
• Gentle on mammalian cells—no cytotoxic effects
• Simple 1:1000 dilution added directly to media
• Works in as little as 2–3 weeks

🧪 Clean your cultures without losing your cells.

🔗 Get AffiCLEAN® MycoX here

The Complete Workflow for Reliable Cell Culture

By combining:

1. Rapid detection (AffiZYME® MycoVisual)
2. Confirmatory qPCR
3. Effective elimination (AffiCLEAN® MycoX)

…labs gain a reliable defense system against mycoplasma contamination. This three-step strategy ensures your research is reproducible, your data is protected, and your cultures stay viable.

Protect Your Results. Standardize Your Lab.

Traditional detection methods like culture or staining are too slow and error-prone for today’s high-throughput environments. With AffiGEN’s mycoplasma control system, you benefit from:

• Speed – 1-hour visual detection + 2-hour qPCR
• Accuracy – qPCR with controls
• Efficiency – cleanup that preserves valuable cell lines

🧫 Don’t let hidden contamination derail your experiments.

🔗 Related Products to Complete Your Workflow

🔍 AffiZYME® MycoVisual Kit (Colorimetric Detection)

🧪 AffiPCR® MycoDetect Kit

🧼 AffiCLEAN® MycoX Mycoplasma Removal Mix